DNA
Part:BBa_K4177001
Designed by: Yuyan Chen Group: iGEM22_HiZJU-China (2022-10-08)
HarmOR10
G protein-coupled receptor (GPCR) HarmOR10 is one of the odorant receptors (ORs) isolated from Helicoverpa armigera, which is specific for benzaldehyde. In the project, we plan to introduce the encoding gene of HarmOR10 from Helicoverpa armigera into engineering yeasts (S. cerevisiae). Simultaneously, the original GPCR of the yeast α-factor receptor (Ste2) will be knocked out. When tea plants infested by tea aphids thus release benzaldehyde into the air, HarmOR10 would recognize the signal and evoke further intracellular GPCR pathways in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
Illegal XhoI site found at 1180 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |